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Our technique relies on a search process that generates variants of the original sample without modifying their semantics.
Alternative splicing of transcripts from promoter #2 generates variants #2 (V2C18) and #3 (V3C18) [7], [7], which are secreted under the control of both liver-specific and ubiquitous transcription factors.
In tumours, aberrant splicing generates variants that contribute to multiple aspects of tumour establishment, progression and maintenance.
The central assumption is that there is a mutation mechanism that generates variants of the code, and a mechanism to select amongst these variants.
Indeed, mutation of the Cyt c axial His residue to Met, Lys, or Arg generates variants that fail to attach heme, further establishing the necessity of His in Cyt c biogenesis.
Due to the high prevalence of E variants in these studies, they took into consideration mostly single nucleotide polymorphisms, as in the E6 gene, which generates variants designated L83V (T to G substitution at 350 nucleotide position).
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Promoter #1 generates variant #1 (V1C18), which is a ubiquitous structural basement membrane component.
The first step involves the generation of new variation by mutation and recombination, whereas the second step determines which randomly generated variants will persist into the next generation.
But these are hard points to prove because researchers can't generate variants or observe naturally occurring ones.
The second part is about generating variants examples of existing type of production systems.
Protein engineering of microbial rhodopsins has been successful in generating variants with improved properties for applications in optogenetics.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com