Unfortunately, this substitution pattern comes at the price of a significant increase in lipophilicity which, when chemically linked to biomolecules, may substantially impact metabolism and biodistribution, generating unspecific uptake and leading to poor PET imaging quality.
The electrophysiological data imply that the sPB1-F2 protein generates an unspecific conductance including permeation by Ca2+ and Cl−.
We reason that the low number of projects performed in the same experimental backgrounds generating the same unspecific signals might be the cause for the lack of correlation between hit and similarity of biological activities of two assays after the application of filter F2.
The latter generates the important interplay of specific interaction and unspecific repulsion which both contribute to the complex process of cell adhesion and interaction.
As controls we used chromatin from wt mice with the anti-GFP antibody a procedure that is superior to other methods, which omit the primary antibody or use an unspecific primary IgG for control, as our approach generates the same background in sample and control.
Together, these data support that stable-bleb cells are able to generate both specific and unspecific traction forces during migration, highlighting their versatile migration competence in various adhesive and non-adhesive environments.
On the other hand, the use of a novel, potentially very sensitive approach, later shown to have pitfalls related to the detection of unspecific bands generated by partially digested PrPSc fragments [ 25], likely led other investigators to overestimate the incidence of the concurrent PrPSc types [ 35, 45].
However, this result does not specify whether olfactory interference generates a specific inhibition concerning only the odor that elicited it, or whether olfactory interference is unspecific (the bee learns to be unresponsive whatever the odor).
Give them general and unspecific information.
Accordingly, cell adhesion is a complex process induced by specific ligand-receptor interactions which compete with unspecific repulsion forces generated by the glycocalyx57,58,59,60, which can be mimicked by inserting lipopolymers38,51.
