The Perl script refold.pl generates single structure predictions using a previously computed consensus structure as constraint.
These clones were subjected to site-directed mutagenesis (either using sequential rounds of mutagenesis with primers generating single codon changes or using primers generating multiple codon changes) using the QuikChange® technique (Agilent Technologies) to produce chimaeric and kinase-dead versions.
In summary, although templating methods can be used to generate single crystal CaCO3 fibers such that the morphology and size is precisely defined by the template the use of additives offers many advantages such as experimental simplicity and facile scale-up.
Random hexamers were used to generate single-strand cDNA using MMLV Reverse Transcriptase according to the manufacturer's protocol (Invitrogen).
A thin gold film semi-transparent heater was designed and used to generate single bubbles.
The methods shown above can be used to generate single strips, but in order to cover a larger area, a method to arrange multiple strips is needed.
The structural refinement data of chromite and K2CrO4 were used to generate single scattering paths for Cr III)–O, Cr VI O, Cr III)–O and Cr Fe [50, 51].
The set-up is used to generate single ellipsoidal bubbles in a water glycerol mixture and to analyze their dynamics and their morphology for Eötvös numbers between 0.8 and 8 and Morton numbers between 10−11 and 10−7.
Geometry-based stochastic channel models, such as the WINNER spatial channel models [3], IMT-Advanced models [4], and COST2100 multiple-input multiple-output (MIMO) models [5], have been proposed in various standards and widely used to generate single- and multi-link channel realizations at the carrier frequency up to 6 GHz with a bandwidth up to 100 MHz.
The same crossing design applied by McAllister [24] was used to generate single flies with X and 4th chromosomes transmitted through a single female meiosis.
In brief, T4 DNA polymerase was used to generate single stranded ends in both fragment and vector, the DNAs hybridized and the mixture transformed into E. coli.
