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From an engineering standpoint, one needs a real-world physical system that reliably generates single photons under ambient conditions.
This method generates single objective sub-problems by transforming all objectives except one of them into constraints.
Mutations leading to drug resistance are generated frequently, since the process of reverse transcription lacks a proofreading mechanism and generates single nucleotide mutations at a high rate (3.4 * 10−5 per nucleotide base per cycle of replication; [1]).
The Perl script refold.pl generates single structure predictions using a previously computed consensus structure as constraint.
DOI: http://dx.doi.org/10.7554/eLife.03553.012 Initiation of replication also transiently generates single stranded DNA at defined locations.
Although MosSCI usually generates single copy inserts, the oxSi91 transgene was a rare event specifically selected for high expression and likely represent the insertion of multiple copies.
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These two motifs cause a substrate to act as a coupled system in which a unidirectional cycle generates single-molecule oscillators, whereas sequestration synchronizes the population by limiting the available enzyme under conditions in which substrate is in excess.
In UDG-based methods, a ribonucleotide U replaces a T in the PCR primers used to add overlapping sequences to the insert and subsequent treatment with UDG enzyme generates single-stranded ends in both the insert and the vector for annealing.
Thus, Irinotecan generates single-stranded DNA breaks, which can lead to overall DNA damage and thereby cell death.
Results: We have developed WGBSSuite, a flexible stochastic simulation tool that generates single-base resolution DNA methylation data genome-wide.
Similar to other polymerases, pol λ generates single-nucleotide deletions more frequently in short homopolymeric repeats than in noniterated sequences.
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