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However, compared to viral-based methods, direct delivery of the Cpf1 RNP has several advantages because it avoids the immunogenicity problems associated with using viruses62,63,64,65,66, is straightforward to manufacture67,68,69,70, and potentially generates low levels of off-target DNA damage35,71.
Ionizing radiation generates low levels of DSBs in cellular DNA, but it induces higher frequencies of many other types of DNA damage and also causes damage to other macromolecules inside the cell.
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NOS3 generally functions to generate low levels of short-lived nitric oxide (NO) by converting L-arginine to citrulline in endothelial tissue (Hong et al., 2007).
Figure 8: Inaccessible long genes generate low levels of antisense transcripts.
"They are naïve, service-intensive users generating low levels of traffic, and Globe would just as soon have nothing to do with them," said Vic Poor, a Globe consultant and investor.
We did experiments that verify how effective this approach is to detect real attacks while generating low levels of false positives, and how good it is in detecting new and stealthy attacks when compared to other works.
High-flow rates of fresh gas help to increase the fraction of inspired oxygen (FiO2) [16], to generate low levels of positive end-expiratory pressure [17], and to decrease physiological dead space by flushing expired carbon dioxide in the upper airways [18].
While pointing out that Mexico is still generating low levels of growth, Castañeda said that the country is poised for higher rates of economic expansion.
Furthermore, HDL treated with heparin generated low levels of β1, but not β2.
In the latter study we found high subtype specificity, generating low levels of cross reactivity after an expanded immunization schedule.
SNAP also induced caspase-like activity within 1 h at doses that generated low levels of nitrite (∼10 µM nitrite after 1 h).
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