Sentence examples similar to generates in frame from inspiring English sources

Inclusion of exon 7a generates in-frame premature termination codons which are inducers of non-sense-mediated mRNA decay (NMD), a surveillance pathway to degrade mRNAs with premature termination codons.

In previous studies, we have demonstrated that forced skipping of this mutant exon by treatment of mdx muscle cells with splicomer oligonucleotides can generate in-frame dystrophin transcripts and restore dystrophin expression.

The presented approach is very versatile for generating in-frame deletions, point mutations or insertions within bacterial chromosomes.

That many substrates genes identified in an earlier genetic screen are longer than 2 kbps [10] may be a result of high probability of generating in-frame fusions of longer genes in the random library used for bacterial two-hybrid screenings.

Eight deletions generating in-frame fusions were also found between PFLU4306 and PFLU4305.

Zebrafish fkrp coding sequence was cloned in front of the egfp coding sequence to generate in-frame fkrp egfp construct and make mRNA for injection.

After sequencing, the inserts were excised by a ClaI/ HindIII digest and ligated into pALX190 to generate in-frame fusions with GFP.

To generate in-frame deletion mutants, overnight cultures of the transconjugants were plated onto LB agar plates containing 10% (wt/vol) sucrose.

Stephens et al. found enrichment for alterations within genes and 29 of these were predicted to generate in-frame gene fusions.

The use of specific antisense oligonucleotides to induce exon skipping and generate in-frame transcripts translated into functional, albeit smaller, protein could be an additional approach for the therapy of diseases caused by mutations generating premature stop codons.

The 5' arm should include the translation start codon of the target gene and cloning should be engineered to generate in-frame fusion with the GFP-Luciferase reporter in pELCV.