We generated an endogenous mAID-Venus-ANAPC4 fusion in a cell line stably expressing TIR1 by CRISPR/Cas9-assisted homologous recombination (Supplementary Figure 4a).
Literature estimates of HSL production rates in V.fischeri indicate that such a sparse layer of individual cells, within a chamber that is flushed at this rate, would not be able to generate an endogenous HSL concentration above ~100 pM [ 20].
In ASL, radiofrequency (RF) pulses are used to magnetically alter the status of water protons in arterial blood with respect to those in stationary tissue water, thereby generating an endogenous intravascular tracer [ 28, 29].
Even in the absence of parental protection, eggs of some insects retain the capacity to generate an endogenous immune response against pathogen challenge [ 9, 14, 15]; this is thought to be one important cause for the low incidence of parental care in insects [ 43, 44].
An experimental acute vessel occlusion (Fig. 2A) results in an immediate vascular response that generates a powerful, endogenous stimulus for collateral growth (i.e. arteriogenesis) in otherwise healthy animals.
This, however, would generate a purely endogenous classification, which we want to avoid.
In conclusion, we have shown that locomotor activity rhythms in C. elegans are generated by an endogenous pacemaker and are sustained under constant environmental conditions.
To determine if GlLa also shares a similar localization pattern in Giardia, one of the four chromosomal copies of GlLa was tagged with 3XHA was generated using an endogenous tagging method as described previously [26] [28].
Circadian rhythms in mammals are generated by an endogenous clock composed of a central clock located in the hypothalamic suprachiasmatic nucleus (SCN) and subordinate clocks in all peripheral tissues.
