Pipasic uses all peptide identifications available, not only unique peptides, and generates a strain-specific quantitative output without resorting to a lower phylogenetic resolution.
Introduction of four genes from C. acetobutylicum (ctfA, ctfB, adc and thiolase (thl)) into E. coli generated a strain capable of producing acetone (Bermejo et al.1998).1998
Among the 18 stopped projects were at least five working on adapting the MERS virus to mice in order to generate a strain that sickens the animals.
Therefore, we generated a strain co-expressing the premnaspirodiene oxygenase of Hyoscyamus muticus (HPO) and the Arabidopsis thaliana cytochrome P450 reductase (CPR) that hydroxylated extracellularly added -valencene.
The results demonstrated that the thermal actuator was able to generate a strain of 5% and a stress of 1 MPa for an input electric energy of 25 J, i.e. a strain comparable to human muscles mechanical properties.
In order to overcome this problem, we have generated a strain that is both hypersensitive to Hsp90 inhibitors as it lacks multiple drug resistance genes, and in which different heterologous and mutant Hsp90s can be expressed by plasmid exchange.
In order to establish this connection, we generated a strain of M. smegmatis, which is devoid of Dps2 gene and named this knockout strain as KK09.
In our approach to reprogram H. polymorpha to produce PEN, we first set out to generate a strain synthesizing enzymatically active ACVS, a 450 kD NRPS.
Detection of the endogenous cyclin C protein was attempted by generating a strain in which an epitope tag was inserted into the endogenous gene.
In this case, we were unable to generate a strain of M. smegmatis with deleted Dps1 and therefore our experiments were limited only to Dps2 deleted M. smegmatis.
Consequently, in order to test this hypothesis, we generated a strain of stably transformed N2a neuroblastoma cells containing the Gal4-luciferaseEGFP γ-secretase reporter system we recently engineered.
