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For example, Drosha, an exonuclease, recognizes a transcript hairpin structure, which it then cleaves and generates a precursor microRNA.
Removal of the signal peptide, spanning residues 1 to 23, generates a precursor with a predicted molecular weight of 66.3 kD.
In every asymmetric division, a type I neuroblast always generates a precursor cell (ganglion mother cell or GMC) that divides once to produce two differentiated cells.
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To generate a precursor, Österberg and colleague Seung-Ho Choi beamed a 100-femtosecond 10-15 ss) pulse of low-energy red light into a 70-centimeter-long tube of distilled water.
In the nucleus, pri-miRNAs are cleaved by the nuclear RNase III enzyme Drosha and cofactor protein DGCR8 (DiGeorge syndrome critical region gene 8) to generate a precursor miRNA (pre-miRNA) (about 70 nucleotides long) [ 7].
During the next division (around nineteen hours APF), the pIIb cell generates a tertiary precursor cell, pIIIb, and a glial cell that enters apoptosis shortly after birth.
Subsequently, these pri-miRNA are processed in the nucleus by a protein complex called "microprocessor," formed by an RNase III called Drosha accompanied by the protein DiGeorge syndrome critical region gene 8 (DGCR8), which recognizes the pri-miRNA and generates a smaller precursor known as pre-miRNA, of between 60 and 100 nucleotides in length, forming a stem-loop structure.
In the nucleolus, RNA polymerase I generates a polycistronic precursor rRNA (35S pre-RNA) that contains the sequences for the mature 18S, 5.8S and 25S rRNA, flanked by the external transcribed spacers (5′-ETS and 3′-ETS) and separated by the internal transcribed spacers (ITS1 and ITS2; Supplementary Figure 1).
The cleavage generates a hairpin-structured precursor of miRNAs (pre-miRNAs) with a size of approximately 70 nucleotides.
The materials were prepared by direct precipitation of ammonium molybdate, ammonium tungstate and nickel nitrate, generating a trimetallic precursor designated NH4-Ni-Mo0.5W0.5-O.
Integration of a selectable marker and template flanked by P-ends would generate a mutagenic precursor for TGC without the need for a preexisting P-element.
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