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NCL is a chemoselective coupling reaction that generates a native peptide bond by a reversible transthioesterification between a peptide fragment containing an N-terminal Cys residue (α-Cys) and another peptide fragment bearing a C-terminal α-thioester group, followed by an irreversible intramolecular N-S acyl shift (Fig. 21).
Interchanging the M6 and α-C regions of H2A with H2A.Z on the nucleosome had only a small effect on SWR1 activity as measured by nucleosomal incorporation of H2A.Z-3xFlag, which generates a native gel mobility upshift.
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Furthermore, an alternative promoter located upstream of exon 6, named Ex1b-e and illustrated in Figure 2A, has recently been discovered to generate a native dominant-negative TCF7L2 protein in embryonic brain neurons [ 77]. Figure 2B depicts the protein structure of TCF7L2 which consists importantly of an N-terminal β-cat binding domain that is lacking in dominant-negative TCF7L2.
This generates a nano-section of the native membrane excised and stabilized by a ring of SMA polymer [ 15].
Significantly fewer sodium ions are adducted to the ions generated from the native-like conformer when native myoglobin is mixed with acid using the theta-glass emitters than to these ions generated from a native solution.
Our method, named as TreeFolder, generates a much higher percentage of native-like decoys than FARNA and BARNACLE, although we use the same simple energy function as BARNACLE.
Additionally, the beating rates among these isolated cell clusters could be synchronized via an electrical stimulation designed to imitate that generated in a native heart.
The same extracts caused high-frequency Ca2+ oscillations when injected into mouse eggs; so these data suggest that CHO cells are not able to generate a response to native sperm PLCζ, even when it is introduced directly by microinjection into their cytoplasm.
We show that coating of decellularized extracellular matrix (DC-ECM) on substrate surfaces is an efficient way to generate a platform mimicking the native ECM environment.
It is considered likely that both the mutation of residue 125 and the modification of the active-site cysteine residue stabilise the structure of the human enzyme, because thousands of attempts to generate a crystal from the native human NAT1 protein have been unsuccessful (A. Kawamura, unpublished results).
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