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Concentrations were calculated using a standard curve generated with specific standards provided by the manufacturer.
Concentrations were calculated by using a standard curve generated with specific standards provided by the manufacturer.
Thus, transgenic lines were generated with specific temporal and spatial reporter gene activity.
PCR linear DNA fragments were generated with specific hybrid primers and high-fidelity thermophilic DNA polymerase (Dynazyme Ext; Finnzymes).
In TCF7L2 knockdown rats (generated with specific TCF7L2 morpholino-oligonucleotides), the process of β-cell regeneration was significantly inhibited.
Furthermore a detailed analysis of the candidate secondary CESAs were performed by functionally characterizing mutants generated with specific artificial microRNA constructs.
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Mice generated with placenta-specific knockdown of specific miRNA using previously reported techniques for placental gene manipulation (Georgiades et al. 2007) may also prove essential in better characterizing the effect of dysregulated miRNA expression on placental function and fetal growth and development.
Alignments generated with EST specific parameters were generally longer [see Additional file 1, Figure S1], which is more appropriate to find homologs to entire RNA transcripts.
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The complete mitochondrial genome of S. nudus was amplified in PCR fragments generated with species specific primer pairs from EST information (see Table 2).
A 582 bp PCR product (generated with the specific primers) with flanking SP6 and T7 promoters was used as a template to generate a DIG labeled ribo-probe complimentary to Awi-GABARAPL2.
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