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There's also a (very crude!) automatically generated transcript below the video box.
The generated transcript expression patterns provide a framework of water calcium-responsive genes in the gill during the initial response after transfer to different [Ca2+].
Moreover, 35% of the expressed genes generated transcript isoforms initiating at iTSSs and significantly stabilized in the absence of Upf1 (see Figure 7A,B).
The generated transcript expression patterns provide a framework of novel water calcium-responsive genes in the gill during the initial response after transfer to different [Ca2+].
TransDecoder [ 50] was used to identify likely coding sequences within Trinity generated transcript sequences after BLASTP (threshold 1e−5) selection with Swiss-Prot database [ 74].
The cDNA-AFLP generated transcript derived fragments (TDFs) were run on an ABI3130xl capillary analyser and visualized with fluorescently labelled probes to accurately estimate their size (see Methods).
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Second strand cDNA synthesis was followed by an in vitro transcription reaction in which biotinylated CTP and UTP were incorporated to the generated transcripts.
The normalized probe intensities were summarized using the generated transcript-specific CDF environment.
The microarray generated transcripts were further mapped to available gene annotation in IPA.
Five regions generated transcripts up to 700 nt in length (data not shown).
The generated transcripts from four developmental stages, infective L3, serum stimulated L3, adult male and adult female, covered 93% of the A. caninum transcriptome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com