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Moreover, a paired-guided RNA CRISPR/Cas9 library has recently been generated to screen for lincRNAs by performing multiplex targeting to delete the entire lincRNA sequences (Zhu et al., 2016).
Products ranging from 200 to 500 base pairs were generated to screen the exons of TERF2IP, TERF2, LRRC29 and FBXL8 (4, 10, 4 and 6 products, respectively).
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Using novel and established experimental strategies side by side, the action of cAMP and cAMP analogs was tested on type Iα PKA holoenzyme, thus generating methodology to screen drug libraries for potential cAMP antagonists with high accuracy, reproducibility as well as potential for automation.
Alerts were automatically generated to remind clinicians that screening was due at a particular well visit.
To achieve this extraordinary density, GMAP features were synthesized at a 5 μm scale, resulting in a 4.84-fold smaller surface area than the 11 μm features on the TRCBC array, a commercially unavailable microarray that was previously generated to perform pooled shRNA screens on a subset of the TRC1 shRNA collection [ 14].
A three-dimensional pharmacophore model of CA IX inhibitors was generated and used to screen the ZINC database of commercially available compounds.
Supplementary Tables S1 and S2, available online at http://dx.doi.org/10.1155/2013/268249, shows design matrix (eight experiments) generated by PB to screen carbon and nitrogen sources.
Labeled primers (supplementary material Table S1) to PCR amplify a 500 bp region surrounding exon 5 of ush1c were generated and used to screen 8448 genomes in a TILLING screen as described (Moens et al., 2008).
Hence, the generated model was used to screen a large set of naturally occurring chemical compounds and predict their biological activity to identify more potent natural compounds targeting mPTPB.
In this study, we describe an approach to integrate the maize physical and genetic maps by using PCR-based STS markers generated from maize sequences to screen BAC DNA pools.
Here, we report a study design where the primary aim is to generate clinically useful methods to screen women to assess their risk of adverse pregnancy outcome.
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