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To provide more accurate synaptic measurements, synaptoneurosome preparations were generated to isolate the synaptic components from the whole homogenate (Fig. 7a).
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For overlapping nuclei, separate projection images were generated to visually isolate the nuclei.
Moreover, from the graphical model analytical redundancy relations are systematically generated to detect and isolate faults in the process.
Dendograms were generated to visualize relationships among the isolates.
The sequences that where unequivocally identified as those of DEF- or GLO-like transcripts were employed to generate specific primers to isolate the 5' end of the sequence with the 5'-RACE kit (Roche).
A mutant virus with a GFP insertion in the US1 gene (α22 protein) was generated and then used to isolate a virus with a mutation, Y116C, in the α22 protein.
Klein et al. [ 26] generated a congenic strain to isolate a QTL for peak bone mineral density on mouse chromosome 11 that was previously identified by Klein et al. [ 45].
Further support for this idea has lately been generated by the ability to isolate and assess the tumour-initiating properties of various cell fractions isolated by fluorescence-activated cell sorting (FACS) based on certain cell surface markers such as CD34, CD44 or CD133 [ 7].
We have established an approach to generate and isolate viable CTCs to study their response to hypoxia.
To isolate 'internally' generated intentions from those impelled by external stimulus, observers are asked to 'choose freely' or to wait for a felt 'urge'urge
However, because the spontaneous occurrence frequencies of LOH and mitotic chromosome loss are less than 1 × 10−4, it is difficult to isolate the generated a- and α-type cells from mixed cell populations (Hiraoka et al. 2000; Kumaran et al. 2013).
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