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This suggests that new piRNAs have been generated to introduced sequences sometime within the approximate 35 mosquito generations that separated the introduction of the transformation plasmids into the germ line and collection for library construction.
piRNAs were also generated to introduced transposons.
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Eventually, using the validated ANN, an abundant number of curves (Master Curves) were generated to introduce a user-friendly equation.
Sequence changes to this region were generated to introduce restriction enzyme sites used for plasmid construction.
Next, two mini-targeting vectors (MTVs) were generated to introduce loxP sites into the retrieved DNA segment.
To allow the insertion of the DNA sequence by homologous recombination, a gap-vector must be previously generated to introduce homologous flanking sequences for the posterior recombination insertion step.
The data release discussion paper, which was prepared to describe the kinds of data that were generated and to introduce the topic of data sharing, read that "any information on ethnic group, geographical location or country linked to individual-level genetic data could potentially provide a wealth of information about the people in that ethnic group, location or country.
Systematically introduced alterations were generated to minimize background expression while maintaining high levels of induced expression.
Indeed, it has often been claimed that one of the quickest ways to improve a country's infrastructure or to generate jobs is to introduce such a tax.
CRISPR/Cas9 has been used successfully in many model organisms to generate mutants or to introduce targeted changes by HDR (Hsu et al. 2014).
To examine the effect of STK33 per se, the STK33 RNAi was generated and introduced into Fadu cell.
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