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We generated the point mutants of these residues alone and in combination in the context of full-length human HOP.
Using the QuikChange strategy (Stratagene), we generated the point mutations of SRSF1 and SRSF9.
SS generated the point mutation in SEC62 and performed Ca2+ imaging experiments with combined knockdown of SEC61A1 and SEC62.
Therefore, we also generated the point mutant PRSS1-E79K and investigated its autoactivation properties in the same way as for wild-type trypsinogen.
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According to this location, we can generate the point spread functions (PSF) for each detector and perform deconvolution for image restoration.
This can be achieved by testing each matrix product in the cartesian basis or directly when generating the point group elements.
(Golparvar-Fard et al. 2011) further reduced the modeling time and cost by generating the point cloud from both photos and SfM.
The algorithm can be considered to find a solution (global optimum or near global optimum) if it can generate the point in the optimality region R0 defined as: R 0 = X ∈ B | f X < Φ + ϵ (15).
The calculation (the same one used to generate the point estimate) is carried out for those values to produce a possible final value of the estimate.
The first stage is to determine and label the landmarks in a set of training images, which will be used to generate the Point Distribution Model (PDM) that describes the target shape.
To generate the point mutation targeting cassette for pSP44, a 450 bp fragment of exon 5 was amplified from pTL2 using primers lat1_03F/lat1_218R introducing to codon change TGT > AGT with the reverse primer.
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