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JG generated the expression analysis.
RK, RJO and VTC generated the expression constructs and the sorted-cell populations.
GW and YW designed the primers and generated the expression constructs.
After scanning, the GeneChip® Operating Software (GCOS; version 1.1) generated the expression data for every single chip.
In the hidden factor approach, for each cluster, we first generated the expression levels of a single controlling factor z by sampling the standard normal distribution.
JY and SI generated the expression plasmid for wild type human NAMPT protein and contributed to the construction of plasmids for mutant proteins.
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All the rules governing management of the cosmos are engraved on the shaft of Wotan's mighty spear, fashioned from the tree that cost him his other peeper, thereby generating the expression "turning a blind eye".
It remains unclear whether these regions make different contributions to a single aversive learning process or represent independent learning mechanisms that interact to generate the expression of pain-related behavior.
Then, pBBR1MCS-2::p lac ::phaC1AB was digested with SspI to generate a 4.7-kbp expression cassette of the phaC1, phaA and phaB1 under control of the lac promoter and ligated with the EcoICRI linearized fragment of pBBR1MCS-2::aldhaTdhaT::pct (Heinrich et al., 2013) to generate the expression vector pBBR1MCS-2::p lac ::aldD::dhaT::pct::p lac ::phaC1AB.
To demonstrate that the 2A site is functional in our construct, the pEnt-Emr/Tet cassette was recombined by the Gateway method into the pDest27 expression vector to generate the expression vector pExp/pD27/pEnt-Emr/Tet (Fig. S2).
Sequencing a 200-bp fragment of the phoP gene in the H37Ra and H37Rv strains utilized in our laboratory, confirmed this SNP to be present in the H37Ra used to generate the expression data presented here (data not shown).
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CEO of Professional Science Editing for Scientists @ prosciediting.com