Sentence examples similar to generated strands from inspiring English sources

We generated strand specific cDNA probes in situ after covalently binding NH2-modified cDNA inserts onto cross-linked N-hydroxysuccinamide slides in a strand specific manner.

Using this protocol, we have generated strand-specific RNA-Seq libraries for parasites throughout the IDC as well as for separately purified nuclear and cytosolic RNA fractions resulting in the sequencing of close to 90 billion nucleotides.

The, non-stranded, Illumina TruSeq kit can be adapted to generate strand specific libraries and can be used to access detailed information on the transcriptome.

We have modified an existing non-stranded automated RNA library protocol into a protocol that generates strand specific libraries by using the Illumina TruSeq kit in combination with other reagents.

Further we show that replication plays a key role in generating strand asymmetry, and that the relative contribution of selection and mutation varies among nucleotides.

To generate strand-specific standard curves for ssqPCR, and strand RNA was transcribed in vitro from a plasmid containing a portion of the nsP1 gene from either ONNV or CHIKV.

It inactivates virus infectivity by generating strand-breaks in the genetic material and has the further advantage, compared with chemical agents, of high penetration into and through biological materials [7].

One of the most reliable design strategies for generating strand-biased siRNAs is to use asymmetric 25/27-nucleotide 25/27-nucleotide].

We also discuss alternative mechanisms that can generate strand biases and involves error prone DNA polymerases and reverse transcription.

With the advent of many strand specific RNA library preparation protocols increasing number of RNA sequencing experiments are generating stranded RNA sequencing data [ 7– 9].

To determine the role of the alignment method in generating strand bias, we aligned our samples using an additional aligner: Bowtie [ 13].