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For tomographic data analysis, three-dimensional regions of interest (ROI) were drawn around the tumor, and the total amount (in picomoles) of fluorochrome was calculated by TrueQuant software (PerkinElmer/VisEn Medical) using previously generated standards of the appropriate dye.
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Plasma LOOH concentration was calculated from the absorbance at this wavelength, against a cumene hydroperoxide generated standard curve.
Amylase concentrations in the samples were determined using the generated standard curve.
The TNF titers in samples were calculated by SPF software using a cytokine standard generated standard curve.
The generated standard curve can be applicable for accurate quantification of PPRV.
For quantification, relative numbers of cells per well were determined using a generated standard curve.
The software generated standard lines that set apart the limbs from the trunk and head.
Data were analyzed against the linear portion of the generated standard curve.
The ODs were interpolated into absolute concentration using Stata 5.0 (StataCorp, USA) generated standard curves.
The generated standard curve covered a linear range of six orders of magnitude of the standard plasmid DNA.
We generated standard vectors that contained the PCR fragments for each mutation and the respective wild-type DNA (Online Resource).
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