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Similarly, the microscopic images of the generated tissue stained immunohistochemically for collagen 1 and osteocalcin were compared with the immunohistochemically stained normal bone samples as a positive control.

The human HNSCC tissue microarray was generated and stained for OPN and IKK-β as previously described [31].

Following skin incubation at air-liquid interface for 24 or 48 hours epidermal sheets were generated and stained for CD207 to identify and quantify LCs populations.

The gata-1GFP fish embryos were fixed in 4% paraformaldehyde/PBS at 4°C overnight, and cryostat sections (10 µm) were then generated and stained using an anti-GFP polyclonal antibody (Wako) (1∶500) and FITC-labeled anti-rabbit antibody (1∶500).

Yeast expressing WNV DIII was generated and stained with mAbs as described previously [ 30].

Serial sections (thickness, 7 μm) were generated and stained with hematoxylin and eosin (HE).

Detergent-extracted parasites were generated and stained as previously described (31).

From each patient block three slides were generated and stained with HER2, Ki-67, and hematoxylin and eosin (H&E).

Sections of 5 mm thickness were then generated and stained with Periodic acid Schiff reagent for subsequent light microscope examination.

Eye discs containing homozygous mutant clones of either rbf1 or cic were generated and stained with dihydroethidium (Dhe) to monitor the levels of ROS.

Enhancer candidate constructs were amplified from mouse genomic DNA, subcloned into the Hsp68-LacZ vector and transgenic mouse embryos were generated and stained (Cyagen Biosciences, Sunvale, CA).