We first generated RIP-Cre, Bcl-xfl/fl mice (and various control genotypes) lacking the oncogene, and aged them out to >12 wks to assess possible effects on β cell development and homeostasis.
Küntscher et al. [ 18] reported that using a tourniquet would be as effective as direct clamping of the femoral artery for generating RIP.
p19Arf−/− mice were backcrossed 8 generations into C57BL/6 and intercrossed with RIP-Tag2 mice (C57BL/6) to generate RIP-Tag2; Arf+/+, Arf+/−, and Arf−/− mice.
In N. crassa, crosses involving Dp strains can generate RIP-induced mutant progeny (Perkins et al. 1997).
In such species, RIP activity must be inferred from the dearth of intact RIP target sites or overrepresentation of the nucleotide combinations generated by RIP, if they are known.
Later, the mature hurricane generated strong rip currents as far away as South Padre Island, Texas, where two swimmers drowned in separate incidents over the Labor Day weekend.
However, it generated strong rip currents and rough waves on the U.S. East Coast which may have resulted in the crowning death of a 12-year-old boy near Corolla, North Carolina on August 28.
The pleiotropic functions of EpCAM can be allocated to the full-length protein, as well as to EpCAM-derived fragments, which are generated upon RIP.
No T-DNA tag was coincident with multiple loci in the genome, even in AT-rich compartments and telomeres, due to sequence divergence generated by RIP acting on repeated copies of TEs.
To restrict transgene expression to the pancreas, we generated pAAV-RIP Luc, which harbors a rat insulin promoter (RIP) instead of the constitutively active CMV promoter (Fig. 1A).
