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The generated ratio was tested using run test in Easy Care PipeSystem (ECPS).
Competitive hybridizations were carried out on oligonucleotide arrays; processing of the expression data generated ratio intensities between samples and control, with positive values corresponding to genes more expressed in myofibers.
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Consequently, four lists of p-values were generated: ratio-based and intensity-based p-values for dataset B1, and ratio-based and intensity-based p-values for dataset B2.
By contrast, kinematic gait analyses generate ratio level data that have frequently been used to characterize walking deficits for MS patients and test the efficacy of treatments designed to improve them.
The water deficit and control condition replicate arrays were combined into their respective intensity profiles, and subsequently used to generate ratio experiments in Rosetta Resolver and analyzed using the software's background correction, normalization, and proprietary ratio error model [ 9].
Single copy gains and losses generate ratios ranging from 1.3 to 1.4 and from 0.6 to 0.8, respectively [ 16].
This relatively new method can be used to determine absolute gene expression levels and is far more accurate than DNA-microarraying, which commonly generates ratio-based data.
To generate ratios and allele frequencies from WES data, we need to align them to the reference genome and identify the SNPs.
For instance, for chromosome 1, four frequencies corresponding to 19,865 SNPs were randomly simulated and used to generate ratios, means, and coefficients of variation, along the lines of what was done with the real dataset.
The experimental design (see above) generated M-ratios (log-ratio of red and green intensities) representing the log ratio of expression in the two strains.
EMCV generated a ratio of 28 in C6 cells and 16 in N2a cells (Fig 2).
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