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We generated protein expression profiles to compare the PhoP regulon of S. Typhi to S. Typhimurium and identified 67 PhoP-dependent proteins in our analysis.
We generated protein expression profiles for four cases each of surgically resected I-GIST and S-GIST using label-free proteomic analysis.
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In the search for diagnostic and therapeutic strategies for lung cancer, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been evinced as a new and promising discovery platform to generate protein expression profiles in search of overexpressed proteins in lung tumors.
For the analysis at the protein level, two-dimensional gel electrophoresis coupled with mass spectrometry (2DE-MS) has been widely used to generate protein expression profiles [ 10, 11].
SELDI is a high through-put technique used to generate protein expression profiles which, in combination with bioinformatics tools to extract information for biomarker discovery, has been essential in identifying novel protein biomarkers.
We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins.
Kaplan Meier curves were generated for protein expression in overall survival.
Linear templates with a T7 promoter and a Kozak sequence were generated for protein expression in wheat germ lysate.
We generated all protein expression constructs using the standard PCR-based cloning strategy and verified the clones through sequencing.
The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics.
The clone collection was successfully used to generate a protein expression clone resource, which was subsequently used to produce proteins for over 72% of the F. tularensis proteome.
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