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A second set (BmH38RwboA, Bm16MRpgm, and Bm16MRgmd) generated protection but less than Rev 1, and the third set (6 mutants) failed to protect.
The first one (Bm16MRwa**, Bm16MRwzm, BmH38RwbkF, BmH38RwbkD, and BmH38Rper) generated protection not significantly different from that provided by Rev 1 (at the dose and by the route used as reference in mice).
Upon testing for protection against pneumococcal disease in multiple mouse models, strong antibody responses following this vaccine generated protection against otitis media, sinusitis, pneumonia and sepsis from a variety of different strains.
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Traditional vaccines generate protection before a person is infected with a virus and ward it off.
IL-12 producing DC has been found to be powerful in generating protection against tuberculosis infection [32], [33].
The following conditions were determined to be optimal to rank R mutants by their ability to generate protection against virulent B. melitensis.
Previously, we have shown that the recombinant TMV vaccines displaying FMDV VP1 epitopes could generate protection in guinea pigs and swine against the FMDV challenge.
However, highly heterogeneous, difficult to manufacture in a consistent and reproducible manner, and a boost injection required to generate protection are obstacles which limited alum in influenza vaccine use [4], [5].
As controls, the recipients of either DC subsets from naïve mice (nCD8+ DC or nCD8− DC) showed comparable levels of bacterial loads with the PBS treated control mice, therefore not generating protection.
Taken together, the results suggest that iCD8+ DC has strong ability to promote type-1 T cell responses to mycobacterial challenge infection, which may be the basis for its strong capacity to generate protection against both systemic and local challenge infections.
Rubble-mound breakwaters are widely used for beach protection and generate shelter zones in the water, although their feasibility diminishes as water depth increases, due mainly to construction costs.
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