Sentence examples for generated primer from inspiring English sources

Sequences of k nucleotides (9- to 15-mers) were extracted from the generated primer contigs.

We generated primer pairs, where possible, for all SSRs from these three organisms.

To quantify histone modifications, we generated primer pairs using Primer3Plus using qPCR settings on both chromosome arms ranging from as close as < 1 kb from the telomere, up to 250 kb from the telomere (Fig. 2a).

The localization of copies of IS 6110 in the 61 clinical isolates was carried out by PCR as previously describe in this work with all the pairs of oligonucleotides of the generated primer collection (Additional file 1: Table S2).

To confirm that the primers generated by our program were capable of producing valid RT-PCR products, we tested 49 genes with three or more of the generated primer pairs (Table 2).

The generated primers for the alleles of HA-1, HA-2, HA-3, HA-8, ACC-2, SP110 and PANE1 specifically amplified the correct allele (Table 2).

We generated primers for these regions based on the O. niloticus and M. zebra BAC assemblies.

These generated primers can be used in PCR reactions for identification of polymorphic loci for genotyping of individuals.

In this study, we have generated primers for more than 200 loci, based on comparisons from ESTs from C. purpureus and the genome of P. patens.

Brid., we have generated primers for exon-primed intron-spanning loci, based on an alignment of expressed sequence tags (ESTs) from C. purpureus to the Physcomitrella patens (Hedw).

Clones were loaded in 384 well plates and sequenced via primer walking, using a combination of automatically generated primers from autofinish [ 23], or manually generated primers from consed [ 24] The clones were sequenced, assembled, and annotated in a semi-automated pipeline involving a database that stored and provided sequencing, primer, and annotation information for every clone.