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The generated pool of DNA templates is analyzed in a cell-free expression screen and the most efficient template is selected for further preparative scale protein production.
The generated pool was able to stably grow at 45°C while the wild type would not tolerate 43°C.
It has been shown that the generated pool of defective viruses encoding truncated or misfolded proteins represents an important source of viral antigens, associated with a strong activation of HIV-1-specific CD8+ cytotoxic T-cells [ 136].
If they are not sufficient to maintain the rate of protein synthesis, or to provide the required amount of ATP needed to sustain metabolic reactions, then cells activate autophagy in order to rapidly degrade the old or burned-out components and reuse the generated pool of biomolecules.
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To fill each role, Foundry emailed a group of qualified workers on Upwork, a huge freelancer site, which generated pools of candidates.
Subsequently, generated pool-specific datasets contained between 10.6 and 15.4 Mb of sequence.
We generated pools of cells expressing shRNAs that target RAS in a dox-inducible fashion.
In Flag immunoprecipitates of two independently generated pools (labelled a and b) of RAW cells stably over-expressing different amounts of wild-type Themis2, Lyn was successfully co-immunoprecipiated in quantities closely mirroring the levels of Themis2 expression.
In cells expressing similar levels of Y660F mutant Themis2, or two independently generated pools of stable BAP transfectants (labelled a and b), no Lyn was detected, consistent with a role for Y660 in the Themis2/Lyn interaction.
We therefore generated pools of either HCT116 or IPC298 cells harboring inducible shRNAs that target, BRAF, CRAF, MEK1, MEK2 and PIK3CA either singly or in various combinations within the same line.
Toward this end, we transfected RCC10 cells with our pVHL30 constructs, selected with puromycin, and generated pools of stable transfectants.
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