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We have generated overlapping pulses to test how robust the proposed system is.
To genetically characterize PARV4 variants infecting the patients, we generated overlapping sets of amplicons spanning the complete genome of PARV4 from the 3 samples.
An initial PCR generated overlapping gene segments containing the desired mutations that were then used as template DNA for another PCR to create a full-length product.
Amplification of the pfatp6 gene was performed by means of five consecutive polymerase chain reactions (PCRs), which generated overlapping fragments, as described elsewhere.
The other sequences were treated as a target one at a time and generated overlapping fragments from 13 to 25 bases long with a near neighbor ΔG energy less than -14.5.
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Choosing to generate overlapping read pairs instead of non-overlapping read pairs can reduce the effective coverage by half.
This entails that the F'-measure is more useful to compare methods that generate overlapping clusters, whereas the F-measure can be used more generally to compare methods that generate both overlapping or non-overlapping clusters or pair-wise coexpression networks.
In all cases the aperture and the step size chosen generate overlapping areas in order to increase the resolution of the components [18].
Our approach builds on preparing libraries with tunable size distributions such that sequencing from both ends generates overlapping reads.
This algorithm provides a sequence alignment function for the entire circular genome and designs a set of primers that generates overlapping PCR amplification for the entire circular genome, e.g. the mt genome.
Non-exclusive clustering algorithms often generate overlapping clusters, sometimes due to noisy input data.
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