Viral load was determined on the basis of a standard curve generated on a stock of HSV-1 strain McIntyre counted by electron microscopy (Advanced Biotechnologies), and the HSV-1 isolates were assayed at least 3 times.
Assuming shared data is actually re-analyzed, one might expect an increase in citations for those trials which generated data on a standard platform (Affymetrix), or released it in a central location or format (SMD, GEO, GEDP)[11].
34 Beyond these facility level resources, estimates of the resources needed to set up and maintain screening programmes were generated, based on a standard procedure 35 and predicted numbers of human and other resources required to implement the programmes (such as four or five administrative posts per million population for notification, coordination, follow-up, and monitoring activities 5 6).
The loans were moneymakers for Countrywide; internal company documents show that the company made gross profit margins of more than 4 percent on such loans, double the 2 percent generated on standard loans backed by the Federal Housing Administration.
As a preliminary approach, we used quartiles of IHC measurements to categorize our population (percent of stained tumor cells for the data generated on standard slides and quick score QS for the TMA data; Additional file 12, Table S10).
We therefore decided to base our comparison on responses generated by a standard computational model, fitted with empirical data, which has been shown to produce realistic responses ('Materials and methods').
The stimuli were generated using a standard PC running E-Prime (version 1.2; Psychology Software Tools, Pittsburgh, PA) on a 19-in.
Monte-Carlo simulations using closed-form dose expressions, like Eqs. 2 and 3, are computationally feasible and can typically be generated within milliseconds on a standard computer.
Real-time PCR values were generated on the basis of standard curves generated for each gene and are presented as fold changes over non-transfected samples.
