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We noted that values of the amount of cGMP generated obtained with the mass spectroscopy read higher than those obtained with the enzymatic assay and this observation has been made consistently in independent in vitro experiments with recombinant proteins (data not shown).
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In our case, randomly generated eigenvalues obtained from the first factor exceeded those obtained from the analysis, which again suggested a one-factor solution to be the most appropriate.
Based on computationally generated data obtained from solving the unforced wake, a low-dimensional model of the wake was developed and evaluated.
Using the software tool MZmine 2, the metabolite profile of the generated mutants obtained by HPLC HRMS was compared, revealing further intermediates.
The entropy generation due to fluid friction and heat transfer in the fluid system is computed and ratio of rate of heat transfer to irreversibility generated is obtained.
All HIC constructs and additional plasmids for expression in mammalian cells were generated or obtained as previously described [12].
Unfortunately, the possible subsequently generated data obtained using the enhanced Trofile assay, which is more sensitive, are not applicable.
All the sequence datasets we generated were obtained by concatenating gene data sets; maximum likelihood analyses of these datasets then proceeded without using the "partitioned analysis" option.
With the best clones generated, we obtained titers equal or greater than what is generated by transient transfection of the packaging constructs (> 5X10E6 transducing units/ml) (Broussau et al. manuscript in preparation).
Sheffield could merely be playing hard to get, knowing that Steinbrenner is perhaps desperate to make a major move quickly to offset the publicity the Red Sox generated in obtaining Schilling.
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