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For some strains, we re-hopped and generated new insertions on different chromosome.
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The X-linked enhancer trap P-element, PGawB, was mobilized to generate new insertions as described previously [31].
Although in modern mammalian genomes most retrotransposon copies reflect ancient insertions rendered incompetent for future transposition, active members exist and promote genetic diversity by generating new insertions [ 10].
Cataloguing transducable sequences will help, but since some somatically acquired L1 insertions can go on to generate new insertions [ 13], such catalogues will never be exhaustive.
Filtering these 43 loci for only full-length elements with intact left monomers (no 5′ truncation) that also have an intact A-tail greater than 20 bp in length, resulted in 28 Alu elements (about 8.7% of the data set) from seven different subfamilies having all the traditional hallmarks of source elements with the potential ability to generate new insertions.
All these insertions generated new transcription termination sites for the genes where they were inserted in, as deduced from the analysis of ESTs collections.
To compare these variants quantitatively with the CMT2B mutations at identical expression levels, we generated new transgenic lines for rab7 Q67L, rab7 T22N and rab7 WT using the same insertion site in the genome.
HBO lost Floyd Mayweather Jr., generated new crop.
A.M.L. generated new reagents.
Transcript analysis also generated new codes.
Finally, TE insertions may also generate new regulatory elements via their insertion that could result in increased expression levels (Chung et al. 2007).
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