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On average, 15.25 million 76 bp reads were generated for each cDNA library (Additional file 2) and 84%% of the reads generated mapped to the MSGI 1.2 assembly.
Across the sequenced genomes, a mean of 87% of the MRSA252 reference genome was called, and 95% of all reads generated mapped to the reference at a mean read depth of 126 (SD, 20).
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HTSeq (Anders et al. 2014) was used to generate mapped read counts for each transcript.
HTSeq was used to generate mapped read counts for each transcript.
We used the BWA paired-end alignment algorithm to generate mapped data sets.
BWA (v0.6.1) [ 44] with default settings was used to generate mapped data files written in SAM format.
MAQ and BWA generate mapping qualities.
We generated maps using generalized additive models, smoothing on longitude and latitude while adjusting for covariates.
Using generalized additive models and GIS, we generated maps of breast, lung and colorectal cancer risk.
Using generalized additive models and GIS, we generated maps of bladder, kidney, and pancreatic cancer risk.
They then built a database and generated maps to assess Muslims' visibility in the city.
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