These could be disparate events that are independent of infection, although it is also possible that epigenetic events are influenced by the parasite to generate infected cell-associated alterations that cannot be altered by drug treatment over 48 h.
Because of the section's great length you will be moving through its hostile terrain for at least a couple of days, in which time the malignant force it generates infects the world beyond the page.
A scrambled sequence control lentivirus was also generated and infected in an identical manner to serve as a control for non-specific effects of the viral infection.
MFG.EGFP.IRES.puro and the retroviral vector MFG.IκBα.IRES.puro, which encodes a supersuppressive mutant form of IκBαM, were generated and infected into gastric cancer cells, as described previously [ 34].
MFG.EGFP.IRES.puro and the retroviral vector MFG.I κB αM.IRES.puro, which encodes an I κB αM, were generated and infected into three gastric cancer cell lines, as described previously (Lee et al, 2005; Cho et al, 2008).
Briefly, RABV G and LASV GPC antigen were generated by infecting BSR cells with either rVSV-GPC (for LASV GPC antigen) or SPBN (RABV G antigen) in Opti-Pro SFM (Gibco®).
Briefly, effector or memory CTL are generated by infecting mice with LCMV and waiting either 8 or >30 days respectively.
Stable cell lines (RKO, HCT116 or DLD1b) overexpressing HOXB13 or TBX18 were generated by infecting these cells with the recombinant retroviruses and selected with puromycin.
CT26 cells expressing DsRed were generated by infecting cells with the lentivirus, pEF1-DsRed-pur, followed by selection in puromycin (1 µg/ml).
Luciferase-expressing cells were generated by infecting SKOV-3 cells with pCLNCX-luciferase retrovirus (SKOV-3-Luc) (a gift from Dr. Steven Libutti, NCI, Bethesda, MD).
