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Sequential ensemble methods are where the base learners are generated sequentially with boosting as a representative, while parallel ensemble methods are where the base learners are generated in parallel, with Bagging as a representative.
HT29 expression data (GSM277543) was originally generated in parallel with the tissue samples from GSE10950 dataset.
The average fluorescence of each bead pellet was measured and compared with calibration curves generated in parallel with standard dilutions to quantify the target analyte.
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Full-length YFP and CFP fusion proteins are then generated in parallel from each clone by digestion with Srf I or Asc I and re-ligation.
Experiments in which the chaperone was added and removed at a specific step were performed as follows: after the indicated incubation, the supernatant was removed by magnetic isolation of the immobilized transcription templates, and replaced with supernatant generated in parallel, but lacking the histone chaperone.
Mock transfectants (clones transfected with vector DNA without insert) were generated in parallel and were designated as controls.
Ct is directly proportional to log10 of the copy number of the input templates with respect to a standard curve generated in parallel.
mRNA expression profiles were generated in parallel to the miRNA data.
This is consistent with the fact that although TK21 and TK24 were generated from S. lividans 66 (John Innes Stock No. 1326) in the same study, they were generated in parallel [ 16].
Control cell lines were generated in parallel using the pLNCX2 empty vector.
To use as a positive control, IPC-specific dilp2 probes were generated in parallel reactions.
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