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Among four rare dTDP-sugars generated in cytosol of engineered strains, YjiC solely transferred l-rhamnose from dTDP-l-rhamnose and tuned to rhamnosyltransferase.
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Besides, H2O2 is generated in cytosol by several oxidases.
The TCA cycle is composed by a set of eight enzymes presented in the mitochondrial matrix cycle coupling the product of the oxidation of pyruvate and malate (generated in the cytosol) to CO2 with the production of NADH by the respiratory chain (Fernie et al. 2004; Millar et al. 2011).
It is also part of a shuttling system in the inner mitochondrial membrane, the malate-aspartate shuttle, ensuring the transport of reduction equivalents generated in the cytosol into the mitochondrion [ 30, 31].
The porphyrin ring structure is generated in the cytosol, and then the haem precursor protoporphyrinogen IX is transported into the intermembrane space of mitochondria.
In any case, once the Δ30 form is generated in the cytosol, it would be hardly transported to the nucleus, because the Δ30 enzyme lacks the first three amino acids of the potential nuclear localization signal.
hmFCCs appear to be generated in the cytosol from less polar precursor-FCCs.
As shown in Figure 2B, COPII vesicles, marked by the reporter protein SEC22B, were generated in the presence of cytosol, membrane and nucleotide.
However, OVA protein was not found in cytosol of γFe2O3/DMSA NPs groups.
Biosynthesis of SAM is catalyzed in cytosol of cell by SAM synthetase using ATP and L-methionine as the substrates.
Reactive oxygen species (ROS) are generated in the mitchondria as by-products of normal aerobic respiration and in the cytosol by oxidases such as NADPH oxidase.
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