When tissues are generated in combination with new biomaterials, it is expected that they develop a high degree of functionality similar to the one known from the respective organism.
Individual BAC sequences were assembled with Phrap[ 18, 19], first with just the BAC generated sequences, then in combination with the WGS reads that overlap the BAC as an enriched BAC (eBAC).
Sequences from each BAC were assembled with Phrap, first with just the BAC generated sequences, then in combination with the WGS reads that overlapped the BAC as an enriched BAC (eBAC).
The approach followed in this paper is based on (i) choice of methods and technologies for data collection, (ii) choice of solution algorithms for solving the vehicle routing problem described in the previous section, (iii) computation of the added value generated by data reliability in combination with the chosen solution algorithms.
The assembly process consisted of multiple phases: First, BACs were assembled with BAC generated sequence, then subsequently in combination with the individual overlapping WGS reads.
In order to generate the character tables, properties of representations are calculated first and the characters for the symmetry operations are generated based on the operations in combination with the representations.
Double labeling of brain sections was performed using the rabbit anti-GPR17 polyclonal antibody (1∶100) generated as previously described [11] in combination with the selected primary antibody, in PBS 0.01 M, 0.1% Triton X-100.
As K2269E is common among the different lineages, we also generated clones containing this mutation in combination with the other three.
Hence, a PCR product generated from these 5' 47-mers in combination with the normal 3' primers, produced fragments shorter than when using the normal 5' primer, as these deletions were left out during sequence extension complementary to the 47-mer.
This allele has been used to generate primary mouse lung tumors in combination with the LSL-Kras G12D allele using an adenovirus expressing both Cre and Flp (Singh et al., 2010).
