11β-HSD1 staining was performed using 11β-HSD1 sheep-derived antibody, generated in-house (39) in combination with donkey anti-sheep secondary antibody (Alexa Fluor 488; Invitrogen, Paisley, UK).
In the meantime, the data generated here, in combination with published data from other developmental time points, has the potential to provide a sound basis for the examination of key hormonal, structural, and other pathways involved in cotton fiber elongation.
One potential explanation for our failure to find 6 of the 16 MHC genes surveyed is that the relatively short contigs generated here, in combination with oligo dT priming, produced a strong 3' bias in the 454 sequencing.
The hurricane caused no structural damage while tropical, although it generated rip currents in combination with other storms that killed one person off the New Jersey coast.
The last step of any barcoding workflow is to use newly generated sequence data in combination with a well-maintained database to positively identify the species in question.
Using a molecular model generated with Macromodel in combination with caDNAno, the positions for EP modification were selected.
Using a molecular model, which was generated with Macromodel in combination with caDNAno, the positions for attaching porphyrins were selected to be on two opposite duplexes.
Individual BAC sequences were assembled with Phrap[ 18, 19], first with just the BAC generated sequences, then in combination with the WGS reads that overlap the BAC as an enriched BAC (eBAC).
Sequences from each BAC were assembled with Phrap, first with just the BAC generated sequences, then in combination with the WGS reads that overlapped the BAC as an enriched BAC (eBAC).
