11β-HSD1 staining was performed using 11β-HSD1 sheep-derived antibody, generated in-house (39) in combination with donkey anti-sheep secondary antibody (Alexa Fluor 488; Invitrogen, Paisley, UK).
Maximum-likelihood and Bayesian trees were similarly generated in a combination of the cytochrome b sequence with a 12S fragment, using the only 20 caprine species in which both 12S and cytochrome b were available at GenBank, and an outgroup.
First, a draping knowledge database is generated in which combinations of seed points and reference angles are evaluated in terms of fiber angle deviation, scrap, ultrasonic cuts and material shear.
Two sets of reciprocal hybrids were generated in each genotypic combination using A. thaliana ecotypes Columbia (Col), C24, and Landsberg erecta (L er) diploid (2X) and isogenic autotetraploids (4X) as parents (Col2, Col4, L er2, L er4, C24-2, and C24-4).
A similar approach was used here, however, since we were unable to identify distinct colonies in the clonal assays containing dox (probably due to the efficient up-regulation of Lhx2 expression in all cells), 96 colonies generated in the factor combination supplemented with dox were randomly picked and expanded individually in medium containing SF, IL-6 and dox.
In the meantime, the data generated here, in combination with published data from other developmental time points, has the potential to provide a sound basis for the examination of key hormonal, structural, and other pathways involved in cotton fiber elongation.
One potential explanation for our failure to find 6 of the 16 MHC genes surveyed is that the relatively short contigs generated here, in combination with oligo dT priming, produced a strong 3' bias in the 454 sequencing.
As no correlation between the parameters has been assumed, the set of parameter combinations generated in this manner will contain some combinations which would have very low probability if the true joint distribution were known.
The authors declare that they are pursuing patents for the methods and uses and, when applicable, the elicitors, inhibitors, and derivatives or their combination generated in the name of CIATEJ AC, with E. Prado Montes de Oca et al., as inventors.
