Sentence examples for generated frozen from inspiring English sources

For studies carried out with the Affymetrix U133A and U133 Plus 2.0 platforms, we used previously generated frozen vectors available as part of the frma package in R [ 20, 21].

Additional fixed tissue was cryoprotected in 20% sucrose at 4°C for 24 hrs and then embedded in OCT to generate frozen tissue blocks.

(C ) Threshold stimulation intensity required to generate freezing alone or freezing followed by an activity burst, during the photostimulation period.

As a control, this procedure was also employed to label CRT present in subcellular fractions generated by freezing and thawing.

After RIAIL strains were generated, we froze a strain copy and collected DNA in parallel from the same culture plates.

Lyophilized silk sponges were generated by freezing a 4% wt/v silk solution at −20 °C and lyophilizing at −80 °C.

Crystal nuclei are generated by freezing, and on thawing these sites grow into crystals that act as cross-linking sites for the polymer.

Colony forming units (CFU) generated from cells frozen with intra- and extracellular trehalose were essentially equivalent in size, morphology, and number (∼91%) to those generated from unfrozen cells.

We further examined c-Myc expression in glioma cancer stem cells using sections generated from acutely frozen human glioma surgical specimens.

Currently, several prognostic microarray gene expression signatures for breast cancer have been generated using fresh frozen material [17] [25] and one of them is being validated in a prospective trial [26].

To systematically explore how RNA quality affects microarray assay performance, a set of rat liver RNA samples with a progressive change in RNA integrity was generated by thawing frozen tissue or by ex vivo incubation of fresh tissue over a time course.