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The platform code is generated from templates.
When the data structure is defined, all PERL [ 10] programs and database tables are generated from templates, i.e. no line of code is programmed manually.
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TAP- or 13myc-tagged versions of ORC subunits and Hat1p were generated from template plasmids pBS1579 and pFA6-13 myc-kanMX6, respectively.
Furthermore, in PCR amplification with primer sets targeting C. ultunense, S. schinkii and T. acetatoxydans, products of the predicted length were generated from template DNA extracted from the experimental reactor on day 442 and day 642 of operation.
siRNA can be chemically synthesized or generated from cDNA templates by RNase III via a technique known as esiRNA, in which gene cDNA with RNA polymerase promoter amplified by PCR is transcribed in vitro, and then its products is digested by RNase III to generate siRNAs that is similar to siRNAs generated in vivo by Dicer.
Again, a set of input files for the simulations was generated from the templates, and the parameter sets were scored by running and analyzing the respective simulations.
Comparison was made between data generated from both templates by the measurement of genotype failure and genotype concordance rates.
PCR products generated from pENTR templates were LR-cloned directly into pcDNA6.2/N-EmGFP-DEST to create chimeric GFP expression clones.
PCR products generated from pDEST templates were BP-cloned into pDONR221, before subsequent LR cloning into pcDNA6.2/N-EmGFP-DEST, to create mutant chimeric GFP expression clones.
Riboprobes were generated from cDNA templates by in vitro transcription with a T3 (ht2cr) or SP6 (Pomc) polymerase, according to the manufacturer's protocol (Promega and Ambion Inc) in the presence of S-labeled UTP.
For chromosomes 29 38 (the D-group), chromosome painting experiments were largely unsuccessful, we believe this was because these chromosome paints were generated from single templates and were comparatively weak, even on chicken metaphases [ 27].
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