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According to this protocol, a second derivative of pMAD called pMADLicM2AC was generated for the inactivation of the LicM2 enzyme.
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With the long-term objective of identifying genes influencing the variation in number of dNFs, in the present study we have determined the prevalence of mechanisms generating LOH responsible for the inactivation of the NF1 gene in these tumors.
The mitochondrial membrane damage can also generate the inactivation of key transporters involved in ion homeostasis.
To generate cassettes for inactivation of the KU70 and KU80 genes, left flanks (LF) and right flanks (RF) of each gene were cloned by yeast recombinational cloning on either side of the resistance marker in the vectors pC-G418-YR and pC-SUR-YR to construct pC-G418-KU70-KO, pC-G418-KU80-KO and pC-SUR-KU80-KO (supplementarytary materials and methods).
Experiments using laboratory model waters showed that free reactive chlorine generated in situ during EC disinfection process was the main disinfectant responsible for the inactivation of microorganisms.
PCR products of LKB1 mutant cell lines were mixed 1 : 1 with the products from wild-type cell lines (H441) to generate heteroduplexes as LKB1 mutant cell lines were previously reported to be homozygous for the inactivation of the gene.
This points to Cdc6 as an alternative target for the inactivation of INK4/ARF in human cancer.
H2O2, hole, and toxicity of Cu2O film were found responsible for the inactivation of E. coli.
Therefore, nitrogen gas plasma might be an efficient method for the inactivation of Stx.
We chose 1st order reaction kinetic as model for the inactivation curve.
Sult1a1 is also responsible for the inactivation of thyroid hormones.
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