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We also generated EST data from Alexandrium using '454' pyrosequencing.
We have generated EST and microarray tools for D. ponderosae.
SWC generated EST sequence data, and SA, NW, AB, PKW, YJ, and JLM analyzed sequence data.
5' and 3' sequences were equally represented in the generated EST database.
After EST sequencing, the next step is to analyze the generated EST data.
The generated EST data of A. spinosum represents a high coverage of the transcriptome.
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JL carried out transcriptome sequencing, generated EST-based dCAPS markers and edited the manuscript.
Additionally, we performed BLAST searches to find mitochondrial genes within the newly generated EST-library of Myzostoma cirriferum.
NKI generated EST-based SSR markers, analyzed of previously reported SSR and IBP markers, constructed linkage map and drafted the manuscript.
The generated ESTs have also been invaluable in generating molecular markers.
These transcripts only account for 8.4% of the generated ESTs, however.
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