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Therefore, we evaluated a model in which each of them has a separate mutation rate using the simulated data generated described in Section 3.1.
Transgenics were generated (described in Methods), by co-injection of landing-site lines with pKC27_ mw derivatives and a helper plasmid encoding the ΦC31 integrase.
The p21, PUMA and Bax biotin-tagged promoter sequences were generated (described in our Materials and Methods) and incubated with our lysed matched clinical samples, allowing the p53 protein to bind to these sequences.
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Thus, the representation created describes system structure, while the histories generated describe system behavior.
The TRH-Gal4 line was generated as described below.
Signals were generated as described in the Experimental section.
The transgenic plants were generated as described previously (Aldemita and Hodges 1996).
Survival curves were generated as described [13].
DD mice were generated as described [22].
HCVpp were generated as described previously [31].
Photo-stimuli were generated as described above.
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