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Results showed that introduction of a hydroxyproline or an alanine moiety, respectively, at position 2 or 7 generated derivatives without significant VPAC2 agonistic activity.
Pluripotency of these cells was further confirmed by embryoid body (EB) and teratoma formation assays which generated derivatives of all three germ layers.
Based on this, we also generated derivatives of UAMS-929 UAMS-17299 and UAMS-1730) in which alsSD expression was enhanced in a sarA mutant.
We evaluated the viability of the generated derivatives on glucose, on acetate and during simultaneous utilization of both carbon sources.
To further explore the relationship between DCD expression and ERBB signaling pathways, we generated derivatives of the MCF-7 estrogen receptor positive and HER2-non-amplified luminal breast cancer cells stably expressing DCD.
For this we constructed a suicide vector with I-SceI sites, named pGE (Supplementary Table 1), and generated derivatives for integration of SAs having VHHs binding GFP (pGE flu-SAgfp) or binding TirM (pGE flu-SAtir).
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The generated derivative mutant (mprF::pUCB300) (Table 3) was verified by PCR for the insertion of the recombinant plasmid pUCB300-mprF within the chromosome leading to inactivated mprF gene.
By changing the substitution at the 7-position from the diethylamino- group to a hydroxy- group, we generated derivative 4, which was more soluble in PBS, had an improved quantum yield (Table 1), and still maintained potency for reversing taxol resistance.
Since the IMP cells described in this study failed to generate derivatives of LM, we reasoned that these cells may differentiate into cell populations derived from IM, such as kidney and gonads.
Combinatorial biosynthesis approaches are being used in antibiotic-producing actinomycetes to generate derivatives with novel sugars in their architecture.
Proteins can also be combined with a wide range of drugs to generate derivatives with tailored pharmacological properties.
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