To minimise series effects, a computerised random numbers table was generated, defining the order of testers, leg tested, testing position and knee joint position.
For each bioreactor, a heat map was generated defining the differences of community structure on the basis of the annealing temperatures selected for PCR.
Standard curves represented the cycle threshold value as a function of the logarithm of the number of copies generated, defined arbitrarily as 1 copy for the nondiluted standard.
For the global phylogeny, a concatenated alignment of all 272,365 core genome SNPs was generated (defined as genome positions conserved in ≥99% of the genomes).
Importantly, the paradigm is also easily expandable and flexible; if new codes or concepts are necessary for novel data not yet covered by the current vocabulary, they can be easily generated, defined, and integrated into the paradigm.
We generated defined mutations in different UHRF1 domains that retained SRA domain-mediated binding to hemimethylated DNA substrate sites, TTD-mediated recognition of H3K9me3 and binding of DNMT1, but did not allow maintenance DNA methylation.
Standard curves represented the cycle threshold (Ct) value as a function of the logarithm of the number of copies generated, defined arbitrarily as one copy for the most diluted standard.
For example, the 'James Lind Alliance' (JLA) priority setting partnerships – formal partnerships of patients, carers and healthcare professionals – generate, define, refine and prioritize unanswered research questions [ 15, 16].
Targeted genome editing using engineered nucleases can reliably generate defined genetic mutations, although the range of off-target lesions caused by these techniques is unclear.
An analysis of the precision of the manufactured geometries and the possibility of generating defined surface qualities are contents of this study.
