Rex1GFP-positive cells plated in serum generate colonies of undifferentiated cells, whereas Rex1GFP-negative cells produce predominantly small, differentiated colonies.
The regenerative capacity of satellite cells was determined based on their ability to quickly (overnight) generate colonies of desmin+/BrdU+ myogenic cells progenitor cells [ 30].
(B) Overview of generated colonies for each colony type separately (C) Percentages of cells expressing either CD2, CD14, CD19 or CD71 (markers for T-cells, myeloid cells, B cells and megakaryocyte/erythrocytes, respectively) as assessed by flow cytometric analysis of HSPCs after 9 days of static or dynamic culture.
One group of cell lines generated colonies with high rates of senescence, while others did not produce appreciable amounts of senescent cells.
Surprisingly, the lowest frequency was from the double positive CD133+/CD15+ cells, only 3 out of 192 of which generated colonies.
Different FA concentrations were added to the cultures, showing that FA decreased the number of generated colonies from all progenitor cell lines.
Mak and colleagues generated colonies from both types of embryo, but the analyses remain too superficial to support the claim that naïve pluripotent cells can be extracted from pre-epithelial avian epiblast.
In the absence of attB, no colonies appeared, suggesting that the generated colonies were due to occurrence of site-specific recombination between attB and attP sites [ Figure 4B, part (ii)].
Nevertheless, as 3 cells were required to generate colonies for isolation in each of these techniques, it could not be definitively determined that these colonies were truly SCD [42].
When analyzed by flow cytometry, 59.4% of CD38- HPP-CFC generated colonies containing erythroid, myeloid and NK cells, whereas 46.3% of CD38+ HPP-CFC generated such colonies (22).
