Moreover, galactose treatment did not generate cleavage of caspase 3, the caspase active form, in contrast with cleavage observed with staurosporine, a classical apoptosis inducer and caspase 3 activator (Fig. 2B).
Hepatocyte apoptosis results in caspase 3 generated cleavage fragment of cytokeratin-18 (CK-18) being released.
As shown in figure 6a, electroporation of EU2dT generated cleavage product after a 10 minute incubation.
Calpain I cleaves purified mHDx-1 in vitro, generating cleavage products of the expected sizes, showing that this protease can act directly on HDx-1 and supporting the site prediction.
A similar strategy was used to generate cleavage-resistant forms of MKK3, MKK4, MKK6 and MKK7.
In most specimens that were used for the study, a similar distribution was observed for both collagenase and cathepsin K-generated cleavage of Col II.
Other studies have demonstrated that MMP-13-generated cleavage of specific chemokines – CCL7 and CXCL12 – leads to reduced chemotactic activity (McQuibban et al., 2001; McQuibban et al., 2002).
In patient sera, the shed extracellular domain of 180 kDa, generated by cleavage of ADAM10/17, is detectable but not the 80 kDa form generated by PC5 or Plasmin.
A mature protein of 237 amino acids is probably generated after cleavage of a 17-residue signal peptide and a 21-amino-acid prepeptide with a predicted molecular mass of 25 kDa and a pI of 6.0.
T-cells epitopes are short linear peptides generated by cleavage of antigenic proteins.
sTREM-1 is generated by cleavage of membrane TREM-1 upon myeloid cell activation [38].
