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In order to control the level of EBOV-GP expression, we generated cell lines expressing the GPs of the four known EBOV subspecies in an inducible fashion.
In view of the great demand for human beta-cells for physiological and medical studies, we generated cell lines derived from human insulinomas which secrete insulin, C-peptide and express neuroendocrine and islet markers.
We generated cell lines that can be induced to express specific EAV replicase proteins and showed that DMVs induced by the transmembrane proteins nsp2 and nsp3 form an RVN and are comparable in topology and architecture to those formed during viral infection.
We generated cell lines stably expressing SERCA and selected two lines for the analysis (Figure 3B).
We generated cell lines containing inducible RNAi that targeted the RPB1 3'UTR.
To analyze the role of MMP7 in these processes, we first generated cell lines resistant to oxaliplatin treatment by exposure of the parental cells to the drug over 5 months.
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Every generated cell line was validated for each expression by WB analysis.
A newly generated cell line was derived from a 44-year old female patient diagnosed with astrocytoma grade IV.
Generating cell lines with defined mutations through homology-directed repair based methods are inefficient and can lead to unwanted insertions and deletions in the target loci.
The committee saw only limited scientific potential in other approaches for generating cell lines that might bypass ethical difficulties (Science, 24 December 2004, p. 2174).
Therefore, generating cell lines stably expressing affinity tagged bait proteins in a broad range of cell types and cell lines is important for identifying PPIs that are dependent on different contexts.
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