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Strains expressing either tagged or untagged versions of Pkh1 and Pkh2 were generated by transforming the following plasmids, which were generous gifts from Jeremy Thorner: for Pkh1 we transformed pAM73; for Pkh2, pAM79 (Casamayor et al, 1999) into a Pil1-GFP-expressing strain; for expression of 3GFP-Pkh1 or 3GFP-Pkh2, we transformed TWY566 with pFR37 or pER3, respectively (Roelants et al, 2002).
The signal-to-noise (S/N) ratio can be generated by transforming the experimental observation.
Transgenic lines were generated by transforming rice with the 35S::OsWRKY11-ox construct or the 35S::OsWRNAi1 RNAi construct.
The warped features are generated by transforming the frequency axis by a suitable warping function (speaker-dependent BLT in our case), the models, on the other hand, are trained on unwarped features.
Various mutants of the D1 protein of photosystem II were generated by transforming a Chlamydomonas reinhardtii strain specifically engineered to lack a 0.4 kb fragment in the psbA gene.
The homologous recombination strain was generated by transforming the recipient strain by the lithium acetate method (Gietz et al. 1992), using 10 µg of Cas9 plasmid (E02-025) containing gRNA-1 and gRNA-3 target sequences (Table 2 and Fig. 1b) and 5 µg of the sequence-optimized fragment encoding scFv (Additional file 1: Figure S2).
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To maintain both populations, senescent cells have to be generated by the transformed cells during passages suggesting that PDGFB induced transformation is subject to stochastic events that have a tendency to disturb the homeostasis of the transformed cells, and then leading to senescence.
We set the expectation value E [ ϕ Γ ll | n = 3, Λ ) ] = 2 Λ ≡ N Γ − 1 as fraction ϵ of the AP axis length, hence Λ = ϵ (z 0 P − z 0 A ) / 2. Pseudo-random numbers distributed according to this probability density (Equation 30) were generated by inverse transform sampling of the cumulative distribution function (31) Φ Γ (l | n = 3, Λ ) = 1 − 6 Λ 2 + 4 l Λ + l 2 6 Λ 2 2 e − l / Λ .
This paper has two main aims: on the one hand, to investigate the predictive possibilities of the yield curve for the Spanish public debt market, using the methodology proposed by Diebold and Li (J Econom 130 337 364, 2006); and on the other hand, to study the capability of generating profits by transforming these yield curve predictions into technical trading strategies.
Subsequently, filtered stimuli were generated by fast Fourier transforming the image and multiplying the Fourier energy with Gaussian filters.
The only one-parameter group of transformations S on L sym that commutes with the action of the affine group is generated by the (Fourier transform H ˆ of the) Hilbert transform H.
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