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Using one of these sltBΔ::riboB Af transformants (MAD3669), we generated by transformation a null sltA allele by replacing the sltA coding region with pyrG Af, as above.
Yeast transformants expressing galactose-inducible human AID/APOBEC proteins were generated by transformation with the appropriate pRS426-derived expression vectors (Christianson et al., 1992) in which C-terminally-FLAG-tagged AID/APOBEC cDNAs flanked by a GAL1 promoter and tADH polyA site had been inserted between the HindIII and XhoI sites.
Recombinant Escherichia coli strains for the production of valuable products are usually generated by transformation with plasmid expression vectors.
Transgenic lines were generated by transformation of the mutant plants with a Sor1 (wild-type) allele or control empty vector.
Strain NDH32 was generated by transformation of chromosomal DNA of NDH31 into WB800, followed by selection for chloramphenicol, neomycin and erythromycin resistance (strain NDH32).
Transgenic plants were generated by transformation with binary vectors.
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Chomsky had argued that only deep linguistic representations are context-free, while surface form is generated by transformations (for example, in English passivization and in question formation) that result in a non-context-free language.
In contrast, luciferase activity was detected in several transformants generated by co-transformation with the plasmid pHRLucP.
Transformants generated by co-transformation with pHRLucP revealed an up to 22 (± 1 -fold induction (EuHR-8, Figure 6D).
Protein extracts of transformants generated by co-transformation with the pHRLucP or pHsp70A-GLuc plasmid were separated by standard SDS-PAGE [ 85] without using thiol reagents.
In extracts of transformants generated by co-transformation with plasmid pHRLucP the luciferase activity increased between 33- and 120-fold compared to algae that were kept at 29°C.
More suggestions(15)
generated by transposition
facilitated by transformation
generated by industry
triggered by transformation
generated by transfer
generated by incorporation
created by transformation
produced by transformation
generated by moves
generated by transforming
generated by unemployment
generated by wind
generated by word
generated by user
generated by demand
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