It has been speculated that ECTR circles are generated by resolution of a t-loop structure (14), and a similar mechanism has been proposed to explain telomere rapid deletion events (30).
Here, we describe a comprehensive analysis of DNA methylation patterns generated by high resolution sequencing of hairpin-bisulfite amplicons of selected single copy genes and repetitive elements (LINE1, B1, IAP-LTR-retrotransposons, and major satellites).
Differences among different expression signatures can be generated by insufficient resolution power of the statistical analysis applied, but also by the use of different reference samples (see Methods for details).
This degree of insight into the catalytic activity can only be obtained by correlating detailed structural information, for example, from electron microscopy, with high-resolution molecular insight generated by super-resolution fluorescence microscopy.
Integrated genome maps became a powerful tool to fill the gaps generated by the low resolution of linkage mapping and the problems of genome sequencing and assembly, providing a more accurate scenario of the genome structure.
However, these structures seem to have been figments generated by the low resolution of light microscopy.
The most detailed, single-base resolution maps are generated by sequencing of genomic DNA treated with sodium bisulfite, which converts unmethylated cytosines to uracils but leaves 5meC unconverted [ 44].
The excitement being generated by the observation of diabetes resolution with bariatric surgery may in time provide other novel approaches to test.
Side chains were modeled from data assembled from an extensive rotamer library generated by systematic clustering of high-resolution structures in the PDB.
This report is presented with hyperlinks to high resolution images, animations and surfaces generated by segmentation of CT images to allow further exploration of the results.
